检测认证人脉交流通讯录
我公司所有抗体均为美国进口，国内分装，现全部低价促销！ 品牌：BioFront
Clone: 4B8
克隆：4B8
Volume: 100 μl (1 μg/μl)
体积：100 μl (1 μg/μl)
Specificity: Recognizes JFH-1 NS5B (genotype 2a)
特异性：识别JFH-1 NS5B (基因型2a)Form: Protein G purified
形式：G蛋白纯化
Origin: Mouse
来源：小鼠
Isotype: IgG2a
同种型：IgG2a
Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)
缓冲液/成分：1X PBS pH 7.2；0.1% 叠氮化钠 (NaN3)
Storage: Short Term (<6 months): 4℃. Long Term (up to 1 year): -20℃ (avoid repeated freeze/thaw)
储存：短期（< 6个月）：4℃ 长期（可达1年）：-20℃（避免反复冻融）
Applications: Western Blot（Best anti-NS5B MAb for Westerns!）
应用：免疫印迹（NS5B最佳免疫印迹单抗）
Working Dilutions: Western Blot- 1:1000-1:5,000
工作稀释度：Western Blot - 1:1000-1:5,000
Cell lysate run on 12% SDS-PAGE and transferred to PVDF. Primary antibody incubation performed for 1-hour at room temperature (overnight incubations also suitable at 4 degrees Celsius).
细胞裂解物在12% SDS-PAGE电泳并转膜（PVDF）。一抗室温孵育1小时（4℃过夜孵育也可）。
References:
参考文献：
Amet T, Byrd D, Hu N, Sun Q, Li F, Zhao Y, Hu S, Grantham A, Yu Q. BST-2 expression in human hepatocytes is inducible by all three types of interferons and restricts production of hepatitis C virus. Curr Mol Med. 2014 Mar;14(3):349-60.
Horwitz JA, Dorner M, Friling T, Donovan BM, Vogt A, Loureiro J, Oh T, Rice CM, Ploss A. Expression of heterologous proteins flanked by NS3-4A cleavage sites within the hepatitis C virus polyprotein. Virology. 2013 Apr 25;439(1):23-33.
Nag A, Robotham JM, Tang H. Suppression of Viral RNA Binding and the Assembly of Infectious Hepatitis C Virus Particles In Vitro by Cyclophilin Inhibitors. J Virol. 2012 Dec;86(23):12616-24.
Ferraris P, Beaumont E, Uzbekov R, Brand D, Gaillard J, Blanchard E, Roingeard P. Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection. Cell Mol Life Sci. 2013 Apr;70(7):1297-306.
Conrad KD, Giering F, Erfurth C, Neumann A, Fehr C, Meister G, Niepmann M. MicroRNA-122 dependent binding of Ago2 protein to hepatitis C virus RNA is associated with enhanced RNA stability and translation stimulation. PLoS One. 2013;8(2):e56272.
Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H. Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation. Nucleic Acids Res. 2009 Oct;37(19):6587-99.
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Item #: HCV-4B8
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