检测认证人脉交流通讯录
我公司所有抗体均为美国进口，国内分装，现全部低价促销！ 品牌：BioFront
Clone： 2E3
克隆：2E3
Volume：100 μl (1 μg/μl)
体积：100 μl (1 μg/μl)
Specificity：Recognizes the helicase domain of JFH-1 NS3 (genotype 2a)
特异性：识别JFH-1 (基因型2a) 的NS3 解旋酶片段
Cross-reactivity：Cross-reactive against genotype 1a
交叉反应：与基因型1a有交叉反应
Form：Protein A purified
形式：A蛋白纯化
Origin：Mouse
来源：小鼠
Isotype： IgG1
同种型：IgG1
Buffer/Composition：1X PBS pH 7.2; 0.1% sodium azide (NaN3)
缓冲液/成分：1X PBS pH 7.2；0.1% 叠氮化钠 (NaN3)
Storage: Short Term (<6 months): 4℃. Long Term (up to 1 year): -20℃ (avoid repeated freeze/thaw)
储存：短期（< 6个月）：4℃ 长期（可达1年）：-20℃（避免反复冻融）
Applications: Western Blot, IF, IP, FACS, and TCID50 assay
应用：免疫印迹（Western），免疫荧光（IF），免疫沉淀（IP），流式细胞分析（FACS）
半数组织培养感染量分析（TCID50 assay）
Working Dilutions: TCID50- 1:1000-1:5000, Western Blot- 1:1000-1:5000, IF- 1:1000, FACS- 1:500
工作稀释度：TCID50- 1:1000-1:5000, Western Blot- 1:1000-1:5000, IF- 1:1000, FACS- 1:500
References:
参考文献：
(1) Jirasko et al. PLoS Pathog. 2010 Dec 16;6(12):e1001233.
(2) Backes et al. J Virol. 2010 Jun;84(11):5775-89
(3) Kaul et al. PLoS Pathog. 2009 Aug;5(8):e1000546
(4) Yang et al. J Virol. 2008 Jun;82(11):5269-78.
Cell lysate run on 12% SDS-PAGE and transferred to PVDF. Primary antibody incubation performed for 1-hour at room temperature (overnight incubations also suitable at 4 degrees Celsius).
细胞裂解物在12% SDS-PAGE电泳并转膜（PVDF）。一抗室温孵育1小时（4℃过夜孵育也可）。
REFERENCES:
参考文献：
Paul D, Hoppe S, Saher G, Krijnse-Locker J, Bartenschlager R. Morphological and biochemical characterization of the membranous hepatitis C virus replication compartment. J Virol. 2013 Oct;87(19):10612-27.
Friesland M, Mingorance L, Chung J, Chisari FV, Gastaminza P. Sigma-1 receptor regulates early steps of viral RNA replication at the onset of hepatitis C virus infection. J Virol. 2013 Jun;87(11):6377-90.
Fridell RA, Valera L, Qiu D, Kirk MJ, Wang C, Gao M. Intragenic complementation of hepatitis C virus NS5A RNA replication-defective alleles. J Virol. 2013 Feb;87(4):2320-9.
Wu X, Robotham JM, Lee E, Dalton S, Kneteman NM, Gilbert DM, Tang H. Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation. PLoS Pathog 2012; 8 (4).
Nag A, Robotham JM, Tang H. Suppression of Viral RNA Binding and the Assembly of Infectious Hepatitis C Virus Particles In Vitro by Cyclophilin Inhibitors. J Virol. 2012 Dec;86(23):12616-24.
Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H. Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation. Nucleic Acids Res. 2009 Oct;37(19):6587-99.
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Item #: HCV-2E3
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