检测认证人脉交流通讯录
丙型肝炎病毒NS3（解旋酶）蛋白片段
Catalog Number: HCV-NS3h
产品目录号：HCV-NS3h
Genotype: 2a
基因型：2a
Form: Purified via Nickel affinity chromatography
形式：镍柱亲和层析纯化
Tag: Expressed with a C-terminal 6X His-tag
标签：C端6X His-tag表达
Origin: E.coli
来源：E.coli
Applications: ELISA、Flow Cytometry、SDS-PAGE、Western blot
应用：酶联免疫吸附实验（ELISA）、流式细胞分析（Flow Cytometry）、SDS-PAGE、免疫印迹（Western blot）
Buffer/Composition: 1X PBS pH 7.2; 1X protease inhibitor cocktail, 1 mM PMSF
缓冲液/成分：1X PBS pH 7.2；1X protease inhibitor cocktail；1 mM PMSF
Volume: 50μl/ 250μl/ 500μl
体积：50μl/ 250μl/ 500μl
Concentration: 2 μg/μl
浓度：2 μg/μl
Storage: Short Term: 4℃ Long Term (up to 1 year): -20℃(avoid repeated freeze/thaw)
储存：短期：4℃ 长期（可达1年）：-20℃（避免反复冻融）
Coomassie stained gel showing purification of recombinant NS3 helicase protein.Bacterial cells expressing the His-tagged protein were lysed (lane 2), pelleted, and the SN (lane 3) was loaded onto Nickel resin. The ~55 kD protein was absent in the resin flow thru (lane 4) as well as column washes (lanes 5 and 6). Elutions with immidazole (lanes 7 and 8) yielded ≥95% pure protein.Lane 1 = marker
考染显示纯化的重组NH3解旋酶蛋白。表达His标签蛋白的菌细胞裂解物（lane 2），颗粒状的SN（lane 3）过镍树脂。~55 kD蛋白在树脂流出液（(lane 4）中消失，同见于柱洗涤液（lanes 5 & 6）。洗脱液连同咪唑（lanes 7 & 8）产生≥95%纯度的蛋白质。Lane 1 = marker
-
Item #: HCV-NS3h & HCV-NS3h-500 & HCV-NS3h-1
-
地址：安徽省合肥市经开区 凤凰国际2号楼703室
电话：86 0551-63840399
传真：86 0551-63843599
邮箱：info@biofrontchina.com
手机：86 13955322696
更多产品请关注BioFront官网： www.biofrontchina.com
-
