供应expedeone标记试剂盒Metal Labeling kits批发  欣博盛供

目前的金属偶联操作步骤复杂且耗时，并可能导致抗体回收率较低。

Expedeon的新产品Lightning-Link Metal Labeling Kits耗时短（可以在30秒内建立金属/抗体标记），操作简单（只需将抗体溶液添加到激活的金属配体即可）。

操作步骤

特点和优势

• 手工操作只需30s
• 1h即可完成标记
• 一次只需10μ或100μg的标记物标记您的抗体
• 无分离步骤，十分抗体回收率
• 温和的化学反应，稳定的偶联物质
• 良好的批间重复性
• 应用范围广

规格

·         1x 10 µg

·         1x 100 µg

10种供选择的金属元素:

139La, 141Pr, 144Nd, 151Eu, 153Eu, 154Sm, 159Tb, 165Ho, 169Tm, 175Lu

应用

• X射线荧光
• 质谱流式细胞术
• 多重离子束成像
• 解离放大镧系荧光免疫分析

应用举例

Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link® 159Tb at 100 µg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link® metal labels at 10 µg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue™ (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link® Metal Labeling kit.