epicentre Cap Analog Trimethylated C06005 5 U

　　产品名称:Cap Analog Trimethylated货号:C06005规格:5 U存储温度:-20℃

　　epicentre Cap Analog Trimethylated C06005 5 U产品描述Most naturally occurring eukaryotic mRNAs and many viral RNAs have a modified nucleotide structure known as a“cap”on their 5′terminus. In vivo, the cap structure is involved in the initiation of protein synthesis and in mRNA processing and stability.1 Many viral RNAs are infectious only when capped, and uncapped RNAs introduced into cells via transfection or microinjection are rapidly degraded by cellular RNases.2,3 Therefore,RNA repared for microinjection intooocytes or for transfection into eukaryotic cells should be capped. In vitro, capped mRNAs are translated more efficiently than uncapped transcripts in rabbit reticulocyte lysate or wheat germ translation systems.4 The trimethylated cap is involved in RNA transport5,6 and RNA translation in nematodes.7 Transcripts synthesized for use in in vitro RNA splicing experiments may also need to be capped.8 RNA Cap Analogs Cat. Nos. C32010, C61025, C06005, and C50110 Anti-reverse cap analogs (ARCAs)*, such as m2 7,3′–OG[5′]ppp[5′]G, were found to result in synthesis of transcripts that were more efficiently translated in vitro since the 3′-O-methyl group only permitted them to be incorporated in the correct orientation9,10,11 (see Figure 1). Capping does reduce RNA yields relative to transcription reactions without diguanosine triphosphate, but with the right conditions and high-quality reagents, a large proportion of the transcripts that are made will be capped. For optimum yields of monomethylated, capped in vitro transcripts, we recommend using the AmpliCap-Max™T7 and T3 High Yield Message Maker Kits. EPICENTRE also offers the MessageMAX™T7 ARCA-Capped Message Transcription Kit which is optimized to produce the highest yield of RNA having an ARCA on the 5′end. These kits contain a convenient Cap/NTP PreMix and yield up to 60μg of RNA perμg of Control Template in a standard 30-minute reaction. Capping efficiencies are up to 80%. EPICENTRE’s RNA Cap Analog preparations can also be used with the AmpliScribe™T7, T3, and SP6 High Yield Transcription Kits. Capping reactions with these kits typically yield about 20-40μg of total RNA with ~80% capping efficiency. Protocols for the synthesis ofcapped RNA using AmpliScribe or conventional transcription reagents are given on page 3.

　　epicentre Cap Analog Trimethylated C06005 5 U产品描述大多数天然存在的真核mRNA和许多病毒RNA在其5'末端具有修饰的核苷酸结构，称为“帽”。在体内，帽结构参与蛋白质合成的启动以及mRNA的加工和稳定性。1许多病毒RNA仅在加帽后才具有感染力，而通过转染或显微注射引入细胞的未盖帽的RNA会被细胞RNase快速降解。2，3因此，用于显微注射入卵母细胞或转染到真核细胞中的RNA应当加盖。在体外，在兔网织红细胞裂解液或小麦胚芽翻译系统中，加帽的mRNA比未加帽的转录本更有效地翻译。4三甲基化帽参与线虫中RNA的转运5、6和RNA的翻译。7合成用于体外RNA剪接实验的转录本也可能需要加帽。8RNA Cap类似物Cat。发现编号为C32010，C61025，C06005和C50110的反反向帽类似物(ARCAs)*，例如m2 7,3'-OG [5'] ppp [5'] G，可以合成由于3'-O-甲基仅允许它们以正确的方向掺入9、10、11(请参见图1)，因此在体外翻译效率更高。相对于没有三磷酸双鸟苷的转录反应而言，加帽的确降低了RNA的产量，但是在适当的条件和高质量的试剂的条件下，大部分制成的转录本都将被加帽。为了获得单甲基化，封端的体外转录本的最佳产率，我们建议使用AmpliCap-Max™T7和T3高产率消息产生试剂盒。EPICENTER还提供MessageMAX™T7 ARCA封顶的消息转录试剂盒，该试剂盒经过优化，可产生最高产量的5'末端带有ARCA的RNA。这些试剂盒包含方便的Cap / NTP PreMix，在标准的30分钟反应中，每微克对照模板可产生高达60微克RNA。封盖效率高达80%。EPICENTRE的RNA帽类似物制剂也可与AmpliScribe™T7，T3和SP6高产量转录试剂盒一起使用。这些试剂盒的加帽反应通常可产生约20-40μg的总RNA，加帽效率约为80%。合成协议封盖效率高达80%。EPICENTRE的RNA帽类似物制剂也可与AmpliScribe™T7，T3和SP6高产量转录试剂盒一起使用。这些试剂盒的加帽反应通常可产生约20-40μg的总RNA，加帽效率约为80%。合成协议封盖效率高达80%。EPICENTRE的RNA帽类似物制剂也可与AmpliScribe™T7，T3和SP6高产量转录试剂盒一起使用。这些试剂盒的加帽反应通常可产生约20-40μg的总RNA，加帽效率约为80%。合成协议第3页提供了使用AmpliScribe或常规转录试剂封端的RNA。

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